Product Description
FtsZ | Procaryotic cell division GTPase (cyanobacterial) | AS07 217 | Agrisera
Immunogen: Whole cyanobacterial (Anabaena PCC 7120) FtsZ protein, UniProt: Q3MC27 overexpressed in E.coli.
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Immunofluorescence (IF), Immunolocalization (IL), Western blot (WB)
Related Products: AS09 413 | Anti-FtsZ1 and 2 | plant cell division protein ftsZ1 and ftsZ2, rabbit antibodiesAS10 715 | Anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibodiesAS13 2651 | Anti-FtsZ2 plant cell division protein ftsZ2, rabbit antibodies
Recommended Dilutions: 1 : 200 (IL) Immunogold-TEM, 1 : 500 (IF), 1 : 2000-1 : 5000 (WB)
Molecular weight: 44, 5 | 50 kDa
Confirmed Reactivity: Cylindrospermopsis raciborskii CS-505, Listeria monocytogenes (weak reaction), Synechococcus elongatus
Predicted Reactivity: Phaeodactylum tricornutum, Prochlorococcus sp.
Not reactive in: higher plants
Additional Information: This antibody can be used as a loading control antibody in cyanobacteria. Immunofluorescence has been done by labelling Synechococcus elongatus cells at 30°C for 2 hours with FtsZ antibodies diluted to 1: 500 in blocking buffer. Detection images can be found in Kabeya et al (2010) .This product can be sold containing ProClin if requested.
Background: FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria as well as in chloroplasts. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules.
Reconstitution: For reconstitution add 50 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Cytoskeleton
Research Area: Cell Biology