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Product Description
GFP | Green fluorescent protein (VENUS) | AS18 4227 | Agrisera
Immunogen: Full length, recombinat VENUS protein, expressed in E.coli, UniProt: P42212
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Format: Lyophilized
Tested Application: Western blot (WB)
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Recommended Dilutions: 1 : 1000 (WB)
Molecular weight: 26 kDa
Confirmed Reactivity: cell lysate overexoressing Venus protein fusion
Predicted Reactivity: N/A
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: N/A
Background: Green fluorescent protein (Venus) from Aequorea victoria (Jellyfish), can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when THR-203 is replaced by Tyr) . It is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer.
Reconstitution: For reconstitution add 50 µl, of sterile water
Storage: Lyophilized antibody can be stored at -20°C for up to 3 years. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: TAIR
Category: GFP/YFP/RFP/CFP
Research Area: Tag Antibodies
