Product Description
HydA2 | Iron-hydrogenase HydA2 | AS09 600 | Agrisera
Immunogen: Recombinant, full length Chlamydomonas reinhardtii HydA-2 UniProt: Q8VZZ0
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Western blot (WB)
Related Products: AS09 514 | Anti-HydA | iron-hydrogenase HydA1/HydA2, rabbit antibodiescollection of antibodies to Chlamydomonas reinhardtiiAlgal protein extraction bufferSecondary antibodies
Recommended Dilutions: 1 : 5000 (WB)
Molecular weight: 53, 7 | 47, 3 kDa (after a signal peptide is cleaved)
Confirmed Reactivity: Chlamydomonas reinhardtii, recombinant HydA2 expressed in E.coli
Predicted Reactivity: Ostreococcus sp. Species of your interest not listed? Contact us
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: HydA2 (505aa) has a calculated MW of 53.7 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP can only be estimated, since the cleavage site is known only from in silico analysis. It has a calculated MW of 47.3 kDa and should run in the gel also according to its size.HydA1 (497aa) has a calculated MW of 53.1 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP has a calculated MW of 47.5 kDa and runs according to its size at about 48 kDa
Background: Iron-hydrogenase HydA2 is catalyzing reversible oxidation of molecular hydrogen. Chlamydomonas the protein is present in low levels of 1 µg/liter of culture. Synonymnes: HYD2
Reconstitution: For reconstitution add 100 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Fermentation
Research Area: Algae