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Product Description
L7/L12 | Ribosomal protein | AS08 331 | Agrisera
Immunogen: Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Immunofluorescence (IF), Western blot (WB)
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Recommended Dilutions: 1: 10 (IF), 1 : 1000 (WB)
Molecular weight: 11.9 kDa (Chlamydomonas reinhardtii), 15.7 kDa (spinach)
Confirmed Reactivity: Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %
Predicted Reactivity: N/A
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.
Background: Ribosomal protein L-30 is a part of 50S ribosomal large subunit, synthesized in chloroplast. Schmidt et al. (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96 (5) :1451-1463
Reconstitution: For reconstitution add 200 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Translation
Research Area: Cell Biology
