Product Description
Lhca2 | PSI type II chloropyll a/b-binding protein | AS01 006 | Agrisera
Immunogen: BSA-conjugated synthetic peptide derived from the Lhca2 protein of Arabidopsis thaliana UniProt: Q9SYW8, Q8LCQ4, TAIR: At3g61470. This sequence is highly conserved in Lhca2 proteins of angiosperms (monocots and dicots) and gymnosperms as well as in At1g19150. This gene codes for the very low expressed Lhca6 protein which also has been denoted as Lhca2*1.
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Total IgG. Protein G purified in PBS pH 7.4.
Format: Lyophilized
Tested Application: Western blot (WB)
Related Products: AS01 011 | A set of 10 plant anti-Lhca and anti-Lhcb antibodies (rabbit antibodies) AS01 011 Chlamydomonas | A set of anti-Lhc antibodies for Chlamydmonas (rabbit antibodies) Available antibodies against pigment-binding proteins- LHCPlant protein extraction buffer
Recommended Dilutions: 1 : 2000-1 : 5000 (WB)
Molecular weight: 27.7 | 24 kDa for Arabidopsis thaliana
Confirmed Reactivity: Arabidopsis thaliana, Arachis hypogaea, Bryopsis corticulans, Colobanthus quitensis Kunt Bartl, Chlamydomonas reinhardti (one Lhca-type), Citrus reticulata, Chromochloris zofingiensis, Cytisus cantabricus (Wilk.) Rchb. F., Hieracium pilosella L, Hordeum vulgare, Lasallia hispanica, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Physcomitrium patens, Pinus banksiana (the higher of the two bands detected at 24 and 30 kDa is not considered to be specific to any Lhc protein), Posidonia oceanica, Prasinoderma sp., Pyramimonas sp. Spinacia oleracea, Syntrichia muralis (Hedw.) Raab, Triticum aestivum, Triticale, Zea mays
Predicted Reactivity: Dicots, Gymnosperms
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: N/A
Background: The light-harvesting protein Lhca2 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca2 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca2 forms a heterodimer (LHCI-680) with Lhca3 that associates with the PSI core close to PsaF and PsaK.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065-3073.
Reconstitution: For reconstitution add 100 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: AT3G61470
Category: LHC
Research Area: Plant Biology, Photosynthesis