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Product Description
PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1 (Oryza sativa) | AS09 507 | Agrisera
Immunogen: KLH-conugated ptide derived from Oryza sativa PIP2-1, UniProt: Q8H5N9
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: ELISA (ELISA), Western blot (WB)
Related Products: collection of antibodies to plasma membrane proteins AS09 504 | Anti-PIP1;3 | aquaporin, plasma membrane intrinistic protein 1-3, rabbit antibodiesAS09 505 | Anti-PIP1s | aquaporin, rabbit antibodiesAS09 506 | Anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1, rabbit antibodiesAS09 508 | Anti-PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5, rabbit antibodiesPlant protein extraction buffer
Recommended Dilutions: 1 : 8000 (ELISA), 1 : 2000 (WB)
Molecular weight: 30, 2 | 26-29 kDa
Confirmed Reactivity: Oryza sativa
Predicted Reactivity: >Oryza sativa
Not reactive in: Horderum vulgare
Additional Information: Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.
Background: PIP2;1 is an aquaporin which facilitate the transport of water and small neutral molecules across cell membrane.
Reconstitution: For reconstitution add 100 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Plasma membrane
Research Area: Cell Biology
