Product Description
Sec21p | Gamma subunit, COP vesicles | AS08 327 | Agrisera
Immunogen: GST fusion of a part of recombinant Sec21 of Arabidopsis thaliana Q0WW26, At4g34450
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Immunofluorescence (IF), Western blot (WB)
Related Products: AS10 683 | Anti-HDEL | endoplasmic reticulum retention signa, (100 µg), monoclonal antibodiesAS10 683-25 | Anti-HDEL | endoplasmic reticulum retention signal, (25 µg), monoclonal antibodiesAS08 325 | Anti-Arf1 | ADP-ribosylation factor 1, Golgi marker, rabbit antibodiesPlant protein extraction bufferSecondary antibodies
Recommended Dilutions: 1 : 1000 (IF), 1 : 1000 (WB)
Molecular weight: 98 kDa
Confirmed Reactivity: Arabidopsis thaliana, Phaeodactylum tricornutum, Zea mays
Predicted Reactivity: Brachypodium distachyon, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Coffea canephora, Eutrema salsugineum, Glycine max, Glycine soja, Hordeum vulgare var. distichum, Medicago truncatula, Oryza sativa, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays, Species of your interest not listed? Contact us
Not reactive in: Nicotiana tabacum, Microsporidia sp.
Additional Information: This antibody can be used as a Golgi marker in immunolocalization and as a marker of COP1 in Western blot.References describing immunolocalization (IF) and (IG) studies:Pimpl et al (2000) . In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12 (11) :2219-36. Ritzenthaler et al. (2002) . Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14 (1) :237-61.
Background: Sec21p is a constituent of the COPI vesicle coatomer.
Reconstitution: For reconstitution add 50 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: AT4G34450
Category: Endomembrane system
Research Area: Cell Biology