Product Description
Sml1 | Suppressor of Mec1 lethality | AS10 847 | Agrisera
Immunogen: KLH-conjugated synthetic peptide derived from known S.cerevisie Sml1 sequence. Gene ID: 854945
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Western blot (WB)
Related Products: anti-rabbit secondary antibodies
Recommended Dilutions: 1 : 1000 (WB)
Molecular weight: 11, 83 | 11-12 kDa
Confirmed Reactivity: Saccharomyces cerevisiae
Predicted Reactivity: Saccharomyces cerevisiae
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: Cell preparation for western blot: cells were harvested by centrifugation (4000 x g, 6 min, 4 °C), Supernatant was discarded and cells were resuspended in 500 µl cold TCA buffer (20 mM Tris, pH 8, 50 mM ammonium acetate, 2 mM EDTA, 1 tablet/10 ml of Complete Mini Protease inhibitor cocktail with EDTA (Roche Diagnostics GmbH) ), 500 µl 0, 5 mm Zirconia/Silica Beads (BioSpec Products, Inc, 11079105z) and 500 µl cold 20 % TCA was added, Samples were vigorously vortexed twice for 30 sec (kept on ice in between), 750 µl from the liquid phase was transferred into a fresh Eppendorf tube, Samples were centrifuged for 10 min (20000 x g, 4 °C), The pellet was resuspended in 300 µl TCA-Laemmli buffer and boiled for 10 min at 100 °C
Background: Sml1 is a ribonucleotide reductase inhibitor involved in regulation of dNTP production. It is regulated by Mec1p and Rad53p during DNA damage and S phase. Synonymes: Yml058w.
Reconstitution: For reconstitution add 50 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: N/A
Research Area: Fungi