Product Description
Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 3 | AS08 351 | Agrisera
Immunogen: Pisum sativum TOC75; Predicted POTRA Domain #3; Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. UniProt: Q43715
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Flow Cytometry (Flow cyt), Immunolocalization (IL), Western blot (WB)
Related Products: AS08 345 | Anti- Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 1, rabbit antibodiesPlant protein extraction bufferSecondary antibodies
Recommended Dilutions: 1 : 500 (Flow cyt), 1 : 100 (IL), 1 : 2000-1 : 100 000 (WB)
Molecular weight: 88 | 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
Confirmed Reactivity: Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum, Physcomitrium patens, Pisum sativum, Spinacia oleracea, Thellungiella salsuginea, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.
Predicted Reactivity: Aegilops tauschii, Ananas comosus, Anthurium amnicola, Capsicum annuum, Catalpa bungei, Cicer arietinum, Cucumis melo, Glycine soja, Gossypium arboreum, Medicago truncatula, Morus notabilis, Nelumbo nucifera, Nicotiana sylvestris, Noccaea caerulescens, Populus trichocarpa, Ricinus communis, Sorghum bicolor, Theobroma cacao, Zostera marina, Vigna radiata, Vitis viniferaSpecies of your interest not listed? Contact us
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: Antibody detects Toc75 POTRA domain 3 as purified protein, in chloroplast fraction and in crude envelope fraction
Background: OEP 75 or Toc75; Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446) ; In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87; Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane; YaeT; outer membrane protein assembly complex, (TIGR03303) ; FhaC; Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]
Reconstitution: For reconstitution add 50 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: AT3G46740
Category: Chaperones/Transporters
Research Area: Plant Biology, Photosynthesis