Product Description
XTH-Xet | XET5 Xyloglucan xyloglucosyl transferase | AS08 319 | Agrisera
Immunogen: Two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: ELISA (ELISA), Western blot (WB)
Related Products: collection of antibodies to cell wall components
Recommended Dilutions: 1 : 5000 (ELISA), 1 : 500 (WB)
Molecular weight: 31, 5 | 33 kDa
Confirmed Reactivity: Hordeum vulgare, Oryza sativa
Predicted Reactivity: Species of your interest not listed? Contact us
Not reactive in: Poplar sp., Zea mays
Additional Information: N/A
Background: Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1, 3;1, 4) -?-d-glucans, are fundamental to cell wall properties. These interactions have been assumed to be non-covalent in nature in most cases. A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1, 3;1, 4) -?-d-glucans. It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.
Reconstitution: For reconstitution add 100 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Agrisera collection
Research Area: Cell Biology