Product Description
Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (?, ?, and ?) are primarily associated with centromeric heterochromatin? However, HP1? and ? also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and contain a conserved amino terminal chromodomain, followed by a variable hinge region and a conserved carboxyterminal chromoshadow domain. The chromodomain facilitates binding to histone H3 trimethylated at Lys9, a histone "mark" closely associated with centromeric heterochromatin. The variable hinge region binds both RNA and DNA in a sequence independent manner. The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases, and the p150 subunit of chromatinassembly factor1 (CAF1). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells. HP1 proteins are subject to multiple types of posttranslational modifications, including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation, suggesting multiple means of regulation.
Biovision | 6148 | CBX5 Antibody DataSheet
Antibody Target: CBX5
Target Alternative Name: CBX5, HP1, HP1A
Tag Line: Polyclonal antibody to detect CBX5 in human, mouse and rat samples.
Category: Epigenetics
Host: Rabbit
Isotype: Rabbit IgG
Species Reactivities: Human, mouse, rat
Immunogen Sequence: Fusion protein of human CBX5
Accession #: P45973
Gene ID: 23468
Appearance: Colorless liquid
Form: Liquid
Concentration: 1 mg/ml
Formulation: 100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Purification: Affinity purified
Application: Western blot, Immunohistochemistry
Positive Control: Western Blot: Various cell lysates
Application And Usages: Western blot: 1:500 1:2000, IHC: 1:50 1:200
Country of Animal Origin: USA
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: Verified
Immunocytochemistry Verified: Not Verified
Immunofluorescence Verified: Not Verified
Immunoprecipitation Verified: Not Verified
FACS Verified:
ELISA Verified: Not Verified
ChIP Verified: Not Verified
Dot Blot Verified:
Flow Cytometry Verified: Not Verified