Species Independent. Bovine albumin and avian histones were tested.
Antibody Dilution:
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purification:
Protein G Purified
Storage Buffer:
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration:
1 mg/ml
Specificity:
Detects proteins containing acetylated lysine residues in ELISA and western blots. Does not detect non-acetylated lysine residues.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
1 µg of SMC-153 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
SpecificityDetects proteins containing acetylated lysine residues in ELISA and western blots. Does not detect non-acetylated lysine residues.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of Analysis1 µg of SMC-153 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.