Synthetic peptide from the mid-protein of Human AKT1 (aa. 100-200)
Immunogen Species:
Human
Applications:
WB, IHC
Isotype:
NA
Species Reactivity:
Human, Mouse
Antibody Dilution:
WB (1:1000); IHC (1:50); optimal dilutions for assays should be determined by the user.
Purification:
Peptide Affinity Purified
Storage Buffer:
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration:
1 mg/ml
Specificity:
Detects 65 kDa. Band is detected at ~65 kDa, due to post translational modifications.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:1000 dilution of SPC-767 was sufficient for detection of TSC2 in 15 µg of mouse Brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization:
Cytoplasm, Nucleus, Cell Membrane
Accession Number:
NP_001014431.1
Gene ID:
207
Swiss-Prot:
P31749
Tissue Specificity:
Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancer
SpecificityDetects 65 kDa. Band is detected at ~65 kDa, due to post translational modifications.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of AnalysisA 1:1000 dilution of SPC-767 was sufficient for detection of TSC2 in 15 µg of mouse Brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Tissue SpecificityExpressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancer