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Product Description
Rabbit Anti-Human AKT1 (pSer473) Polyclonal
Product Videos
Size
Size 100 µl
Target AKT1 (pSer473)
Conjugate ATTO 633
Research Area Cell Signaling | Protein Phosphorylation | Serine / Threonine Kinases | PKB / AKT | Cancer | Apoptosis | Cancer | Cancer Metabolism | Cancer | Cell Cycle | PI3k/AKT Pathway
Alternative Name RAC-PK-alpha Antibody, Protein kinase B alpha Antibody, RAC Antibody, Proto-oncogene c-Akt Antibody, Protein kinase B Antibody, RAC Alpha Antibody, AKT Antibody, EC:2.7.11.1 Antibody, AKT 1 Antibody, PRKBA Antibody, AKT1_HUMAN Antibody, AKT1 Antibody
Category Antibodies
Product Type Polyclonal Antibody
Immunogen Synthetic phospho-peptide from human AKT1 around the phosphorylation site of serine 473 (pSer473).
Immunogen Species Human
Applications WB, IHC
Host Rabbit
Isotype IgG
Species Reactivity Human, Mouse, Rat
Antibody Dilution WB (1:250-1:500); IHC (1:25-1:50); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Storage Buffer PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
Concentration N/A
Specificity Detects endogenous levels of AKT1 only when phosphorylated at serine 473 (pSer473).
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis A 1:500 dilution of SPC-1340 was sufficient for detection of AKT1 (pSer473) in 10 µg of human cervical cancer cell line (hela) lysate by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.
Cellular Localization Nucleus, Plasma Membrane, Cell Membrane, Cytoplasm, Cytoskeleton, Ciliary Basal Body, Microtubule, Spindle, Cytosol, Mitochondrion, Nucleoplasm, Cell-Cell Junction, Postsynapse, Protein Complex, Vesicle
Tissue Specificity Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancer
Accession Number NP_001014431.1
Gene ID 207
Swiss-Prot P31749
Field of Use For in vitro research use only.
