WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification:
Protein G Purified
Storage Buffer:
PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Concentration:
1 mg/ml
Specificity:
Detects ~85kDa. No cross-reactivity against phosphor S751-Ataxin-1. Minimal cross-reactivity against S751A mutant of Ataxin-1 by ELISA and immunofluorescence and negative by immunoblot.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
1 µg/ml of SMC-475 was sufficient for detection of Ataxin 1 in 20 µg of COS cells transiently transfected with Ataxin-1 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization:
Cytoplasm | Nucleus
Accession Number:
NP_001186233.1
Gene ID:
20238
Swiss-Prot:
P54254
Field of Use:
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
SpecificityDetects ~85kDa. No cross-reactivity against phosphor S751-Ataxin-1. Minimal cross-reactivity against S751A mutant of Ataxin-1 by ELISA and immunofluorescence and negative by immunoblot.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of Analysis1 µg/ml of SMC-475 was sufficient for detection of Ataxin 1 in 20 µg of COS cells transiently transfected with Ataxin-1 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular LocalizationCytoplasm | Nucleus
Accession NumberNP_001186233.1
Gene ID20238
Swiss-ProtP54254
Field of UseNot for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.