A phospho-specific peptide corresponding to residues surrounding Thr287 and Thr288 of human Aurora A (AA285-291)
Immunogen Species:
Human
Applications:
WB, AM
Host:
Rabbit
Isotype:
N/A
Species Reactivity:
Human
Antibody Dilution:
WB (1:250); optimal dilutions for assays should be determined by the user.
Purification:
Peptide Affinity Purified
Storage Buffer:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Concentration:
N/A
Specificity:
Detects ~48kDa.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:250 dilution of SPC-919 was sufficient for detection of Aurora A (pThr287 + pThr288) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization:
Cytoplasm, Cytoskeleton
Tissue Specificity:
Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
Certificate of AnalysisA 1:250 dilution of SPC-919 was sufficient for detection of Aurora A (pThr287 + pThr288) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular LocalizationCytoplasm, Cytoskeleton
Tissue SpecificityHighly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.