A phospho-specific peptide corresponding to residues surrounding Ser821 and Tyr823 of human c-Kit (AA818-827)
Immunogen Species:
Human
Applications:
WB, AM
Host:
Rabbit
Isotype:
N/A
Species Reactivity:
Human
Antibody Dilution:
WB (1:250); optimal dilutions for assays should be determined by the user.
Purification:
Peptide Affinity Purified
Storage Buffer:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Concentration:
N/A
Specificity:
Detects 109.865 kDa.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:250 dilution of SPC-1003 was sufficient for detection of c-Kit (Ser821+ Tyr823) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization:
Cell Membrane
Tissue Specificity:
Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal
Certificate of AnalysisA 1:250 dilution of SPC-1003 was sufficient for detection of c-Kit (Ser821+ Tyr823) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular LocalizationCell Membrane
Tissue SpecificityIsoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal