Product Description
Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can fuse with proteins of interest without interfering significantly with their assembly and function.
Biovision | 3999 | GFP Antibody DataSheet
Antibody Target: GFP
Target Alternative Name: Green Fluorescent Protein
Tag Line:
Category: GFP
Host: Rabbit
Isotype: Rabbit IgG
Species Reactivities: All species
Immunogen Sequence: Recombinant EGFP
Accession #: ABG78037
Gene ID: 110612126
Appearance: Colorless liquid
Form: Liquid
Concentration: 0.2 mg/ml
Formulation: 30 µg (0.2 mg/ml) affinity purified rabbit anti-GFP polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification: Affinity purified
Application: Western blot, Immunoprecipitation
Positive Control:
Application And Usages: Western blotting (0.5-4 µg/ml) and in immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody reacts with GFP, and its variants EGFP, RFP, YFP, and CFP, etc. Recombinant EGFP (Cat.# 4999-100), RFP (Cat.# 4997-100), YFP (Cat.# 4998-100) and CFP (Cat.# 4996-100) can be used as positive controls.
Country of Animal Origin: China
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: Verified
Immunocytochemistry Verified: Not Verified
Immunofluorescence Verified: Not Verified
Immunoprecipitation Verified: Not Verified
FACS Verified: Not Verified
ELISA Verified: Not Verified
ChIP Verified: Not Verified
Dot Blot Verified:
Flow Cytometry Verified: Not Verified