WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification:
Protein G Purified
Storage Buffer:
PBS pH7.2, 50% glycerol, 0.09% sodium azide
Concentration:
1 mg/ml
Specificity:
Detects ~98kDa. Does not detect human HSP90, Grp74, or GrpE from E.coli.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
0.5 µg/ml of SMC-105 was sufficient for detection of Grp94 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Research AreaCancer, Heat Shock, Cell Signaling, Protein Trafficking, ER Proteins, Tags and Cell Markers, Organelle Markers, Cancer, Cancer Metabolism, Response to Hypoxia, Cell Signaling, Metabolism, Metabolism processes, Hypoxia
SpecificityDetects ~98kDa. Does not detect human HSP90, Grp74, or GrpE from E.coli.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of Analysis0.5 µg/ml of SMC-105 was sufficient for detection of Grp94 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.