Recombinant fragment corresponding to amino acids 329-530
Immunogen Species:
Mouse
Applications:
WB, IHC, ICC/IF, ELISA
Isotype:
IgG1
Species Reactivity:
Human, Mouse, Rat, Bovine
Antibody Dilution:
WB (1:1000), IHC (1:100), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Purification:
Protein G Purified
Storage Buffer:
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration:
1 mg/ml
Specificity:
Detects ~116kDa. Specific for HIF1Alpha.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
1 µg/ml of SMC-184 was sufficient for detection of HIF1α in 20 µg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
SpecificityDetects ~116kDa. Specific for HIF1Alpha.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of Analysis1 µg/ml of SMC-184 was sufficient for detection of HIF1α in 20 µg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.