Product Description
The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
Biovision | 3623 | Histone H3 Antibody DataSheet
Antibody Target: Histone H3
Target Alternative Name: H3F3A , 601128 , Q66I33 , H3.3A , H3F3 , mgC87782 , mgC87783 , 3021 , H3F3B , 601058 , P84243 , H3.3B , H3F3A
Tag Line:
Category: Hsitone
Host: Rabbit
Isotype: Rabbit IgG
Species Reactivities: Human, mouse, rat
Immunogen Sequence: Synthetic peptide near the C-terminus of human Histone H3
Accession #: CAB02546
Gene ID: 8351
Appearance: Colorless liquid
Form: Liquid
Concentration: 0.5 mg/ml
Formulation: 0.5 mg/ml affinity purified rabbit anti-Histone H3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.5 mM EDTA and 0.03% proclin
Purification: Affinity purified
Application: Western blot, Immunohistochemistry
Positive Control:
Application And Usages: Western blotting (0.5-4 µg/ml)and Immunohistochemistry (20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 17-20 kDa histone H3 protein. It does not cross-react with other histones.
Country of Animal Origin: China
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: Verified
Immunocytochemistry Verified: Not Verified
Immunofluorescence Verified: Not Verified
Immunoprecipitation Verified: Not Verified
FACS Verified: Not Verified
ELISA Verified: Not Verified
ChIP Verified: Not Verified
Dot Blot Verified:
Flow Cytometry Verified: Not Verified