A 1:1000 dilution of SPC-1259 was sufficient for detection of HMGB1 in 10 µg of Jurkat, HepG2, COLO, Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Research AreaCell Signaling | Epigenetics and Nuclear Signaling | Transcription | Domain Families | Hmg box
Alternative NameAmphoterin Antibody, Chromosomal protein nonhistone HMG1 Antibody, High mobility group 1 Antibody, High mobility group protein B1 Antibody, HMG-1 Antibody, HMG3 Antibody, HMGB1 Antibody, HMGB1_HUMAN Antibody, SBP 1 Antibody, Sulfoglucuronyl carbohydr
CategoryAntibodies
Product TypePolyclonal Antibody
ImmunogenSynthesized peptide derived from internal of human HMGB1.
Immunogen SpeciesHuman
ApplicationsWB, ELISA
HostRabbit
IsotypeIgG
Species ReactivityHuman, Mouse, Rat
Antibody DilutionWB (1:1000), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
SpecificityDetects endogenous levels of total HMGB1 protein.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of AnalysisA 1:1000 dilution of SPC-1259 was sufficient for detection of HMGB1 in 10 µg of Jurkat, HepG2, COLO, Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.