WB (1:1000), ICC/IF (1:100), FACS (1:250); optimal dilutions for assays should be determined by the user.
Purification:
Protein G Purified
Storage Buffer:
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration:
0.5 mg/ml
Specificity:
Conformation-specific antibody for membrane-bound HSP70. Binds to cell surface HSP70 without permeabilization of cell membrane in tumor cell lines. Does not bind to intracellular HSP70 at low concentrations. Cross-reacts with HSC70. Detects a ~70 kDa
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:250 dilution of SMC-249 was sufficient for detection of cell surface HSP70 in HCT116 cells using Fluorescence-activated cell sorting, applied in flow cytometry, with FITC as the fluorescent probe.
Cellular Localization:
Cell membrane
Accession Number:
NP_005336.3
Gene ID:
3303
Swiss-Prot:
P0DMV8/P0DMV9
Field of Use:
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
SpecificityConformation-specific antibody for membrane-bound HSP70. Binds to cell surface HSP70 without permeabilization of cell membrane in tumor cell lines. Does not bind to intracellular HSP70 at low concentrations. Cross-reacts with HSC70. Detects a ~70 kDa
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of AnalysisA 1:250 dilution of SMC-249 was sufficient for detection of cell surface HSP70 in HCT116 cells using Fluorescence-activated cell sorting, applied in flow cytometry, with FITC as the fluorescent probe.
Cellular LocalizationCell membrane
Accession NumberNP_005336.3
Gene ID3303
Swiss-ProtP0DMV8/P0DMV9
Field of UseNot for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.