Human, Mouse, Rat, Amphibians, Chicken, Fish, Yeast, Yeast (Saccharomyces cerevisiae), Fruit Fly (Drosophila melanogaster)
Antibody Dilution:
WB (1:5000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification:
PEG Purified
Storage Buffer:
PBS pH7.2, 50% glycerol, 0.09% sodium azide
Concentration:
1 mg/ml
Specificity:
Detects ~70kDa. May detect HSP70, HSC70, and HSP72.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
0.2 µg/ml of SMC-163 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgM:HRP as the secondary antibody.
SpecificityDetects ~70kDa. May detect HSP70, HSC70, and HSP72.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of Analysis0.2 µg/ml of SMC-163 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgM:HRP as the secondary antibody.