WB (1:1000), IHC (1:10000), ICC/IF (1:1000), FACS (1:1000); optimal dilutions for assays should be determined by the user.
Purification:
Protein G Purified
Storage Buffer:
PBS pH7.4, 50% glycerol, 0.1% sodium azide
Concentration:
1 mg/ml
Specificity:
Detects ~70kDa. Does not cross-react with HSC70 (HSP73).
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
1 µg/ml of SMC-100 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
SpecificityDetects ~70kDa. Does not cross-react with HSC70 (HSP73).
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of Analysis1 µg/ml of SMC-100 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.