Synthetic peptide conjugated to KLH. Peptide sequence specific to C-terminal part of salmonid HSP70. The target is not present in any other HSP70 proteins.
WB (1:5000); optimal dilutions for assays should be determined by the user.
Purification:
Rabbit Antiserum
Storage Buffer:
Lyophilized rabbit Antiserum. For reconstitution add 100 µl of sterile water.
Concentration:
NA
Specificity:
Detects ~70kDa. It does not cross react with HSC70. It does not detect HSP70 in other species.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
0.2 µl/ml of SPC-313 was sufficient for detection of HSP70 in 20 µg of heat shocked (25°C) rainbow trout cytoplasmic fraction lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
ImmunogenSynthetic peptide conjugated to KLH. Peptide sequence specific to C-terminal part of salmonid HSP70. The target is not present in any other HSP70 proteins.
Immunogen SpeciesSalmon
ApplicationsWB
IsotypeNA
Species ReactivitySalmon, Brook Trout (Salvelinus fontinalis), Rainbow Trout (Oncorhynchus mykiss), Three-spined stickleback (Gasterosteus aculeatus), Fish
Antibody DilutionWB (1:5000); optimal dilutions for assays should be determined by the user.
PurificationRabbit Antiserum
Storage BufferLyophilized rabbit Antiserum. For reconstitution add 100 µl of sterile water.
ConcentrationNA
SpecificityDetects ~70kDa. It does not cross react with HSC70. It does not detect HSP70 in other species.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of Analysis0.2 µl/ml of SPC-313 was sufficient for detection of HSP70 in 20 µg of heat shocked (25°C) rainbow trout cytoplasmic fraction lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.