Synthetic peptide conjugated to KLH. Peptide sequence specific to C-terminal part of salmonid HSP70. The target is not present in any other HSP70 proteins.
WB (1:1000), IHC (1:100), IP (5µg); optimal dilutions for assays should be determined by the user.
Purification:
Protein A Purified
Storage Buffer:
Lyophilized rabbit Antiserum.
For reconstitution add 200 µl of sterile water.
Concentration:
NA
Specificity:
Detects ~70kDa. It does not cross react with HSC70. It does not detect HSP70 in other species.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
1 µg/ml of SPC-314 was sufficient for detection of HSP70 in 20 µg of heat shocked (25°C) salmon cytoplasmic fraction lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
ImmunogenSynthetic peptide conjugated to KLH. Peptide sequence specific to C-terminal part of salmonid HSP70. The target is not present in any other HSP70 proteins.
Antibody DilutionWB (1:1000), IHC (1:100), IP (5µg); optimal dilutions for assays should be determined by the user.
PurificationProtein A Purified
Storage BufferLyophilized rabbit Antiserum.
For reconstitution add 200 µl of sterile water.
ConcentrationNA
SpecificityDetects ~70kDa. It does not cross react with HSC70. It does not detect HSP70 in other species.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of Analysis1 µg/ml of SPC-314 was sufficient for detection of HSP70 in 20 µg of heat shocked (25°C) salmon cytoplasmic fraction lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.