KLH-conjugated synthetic peptide chosen from a highly conserved region of HSP90 found in both the alpha and beta form. The taget peptide is perfectly conserved in animals.
Immunogen Species:
Salmon
Applications:
WB
Host:
Rabbit
Isotype:
N/A
Species Reactivity:
Human, Fish, Salmon
Antibody Dilution:
WB (1:5000); optimal dilutions for assays should be determined by the user.
Purification:
Rabbit Antiserum
Storage Buffer:
Lyophilized rabbit Antiserum. For reconstitution add 100 µl of sterile water.
Concentration:
N/A
Specificity:
Detects ~90kDa. In salmonid fish a cross-reactive band at 40kDa is observed. Antibody will also detect a Human recombinant HSP90 protein.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
0.2 µl/ml of SPC-316 was sufficient for detection of HSP90 in 10 µg Heat shocked (25°C) rainbow trout nuclear fraction lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
ImmunogenKLH-conjugated synthetic peptide chosen from a highly conserved region of HSP90 found in both the alpha and beta form. The taget peptide is perfectly conserved in animals.
Immunogen SpeciesSalmon
ApplicationsWB
HostRabbit
IsotypeN/A
Species ReactivityHuman, Fish, Salmon
Antibody DilutionWB (1:5000); optimal dilutions for assays should be determined by the user.
PurificationRabbit Antiserum
Storage BufferLyophilized rabbit Antiserum. For reconstitution add 100 µl of sterile water.
ConcentrationN/A
SpecificityDetects ~90kDa. In salmonid fish a cross-reactive band at 40kDa is observed. Antibody will also detect a Human recombinant HSP90 protein.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of Analysis0.2 µl/ml of SPC-316 was sufficient for detection of HSP90 in 10 µg Heat shocked (25°C) rainbow trout nuclear fraction lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.