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Product Description
Rabbit Anti-Human IkB epsilon (pSer22) Polyclonal
Product Videos
Size
Size 100 µl
Target IkB epsilon (pSer22)
Conjugate Unconjugated
Research Area Cell Signaling | Epigenetics and Nuclear Signaling | Nuclear Signaling Pathways | NFkB Pathway | Immunology | Innate Immunity | TLR Signaling | Cell Signaling | Epigenetics and Nuclear Signaling | Transcription | Transcription Factors
Alternative Name IκB-ε Antibody, IkB-epsilon Antibody, NFKBIE Antibody, IkappaBepsilon Antibody, IKBE Antibody, IkB-E Antibody, NF-kappa-BIE Antibody, I-kappa-B-epsilon Antibody, NF-kappa-B inhibitor epsilon Antibody
Category Antibodies
Product Type Polyclonal Antibody
Immunogen Synthetic phospho-peptide from human IkB epsilon around the phosphorylation site of serine 22 (pSer22).
Immunogen Species Human
Applications WB, IHC, ICC/IF, ELISA
Host Rabbit
Isotype IgG
Species Reactivity Human, Mouse, Rat
Antibody Dilution WB (1:250-1:500); IHC (1:25-1:50); ICC/IF (1:50-1:250); ELISA (1:20000); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Storage Buffer PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
Concentration N/A
Specificity Detects endogenous levels of IkB epsilon only when phosphorylated at serine 22 (pSer22).
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis A 1:500 dilution of SPC-1392 was sufficient for detection of IkB epsilon (pSer22) in 10 µg of human jurkat cell lysates by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.
Cellular Localization Cytoplasm, Cytosol, Golgi Apparatus, Nucleus, Fibrillar Center, Perinuclear Region Of Cytoplasm
Tissue Specificity Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
Accession Number NP_004547.2
Gene ID 4794
Swiss-Prot O00221
Field of Use For in vitro research use only.
