Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4.
Immunogen Species:
Mouse
Applications:
WB, IHC, ICC/IF
Host:
Mouse
Isotype:
IgG2a
Species Reactivity:
Human, Mouse, Rat
Antibody Dilution:
WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification:
Protein G Purified
Storage Buffer:
PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Concentration:
1 mg/ml
Specificity:
Detects ~60kDa.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
1 µg/ml of SMC-461 was sufficient for detection of LGI1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Certificate of Analysis1 µg/ml of SMC-461 was sufficient for detection of LGI1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.