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Product Description
p53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression thro?gh the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.
Biovision | 3036R | p53 Antibody DataSheet
Antibody Target: p53
Target Alternative Name: TP53, TRP53, P53, LFS1, Tumor suppressor p53, Phosphoprotein p53
Tag Line:
Category: p53
Host: Rabbit
Isotype: Rabbit IgG
Species Reactivities: Human, mouse, rat, dog, rabbit
Immunogen Sequence: Synthetic peptide corresponding to residue surrounding amino acid 375 of human p53
Accession #: EAW90140
Gene ID: 7157
Appearance: Colorless liquid
Form: Liquid
Concentration: 0.5 mg/ml
Formulation: In phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.
Purification: Affinity purified
Application: Western blot
Positive Control:
Application And Usages: Western blot analysis (0.5-4 µg/ml). It detects p53 in samples of human, mouse and rat origins. Other applications have not been determined.
Country of Animal Origin: China
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: Verified
Immunocytochemistry Verified: Not Verified
Immunofluorescence Verified: Not Verified
Immunoprecipitation Verified: Not Verified
FACS Verified: Not Verified
ELISA Verified: Not Verified
ChIP Verified: Not Verified
Dot Blot Verified: Not Verified
Flow Cytometry Verified: Not Verified
