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Product Description
Rabbit Anti-Human PDK1 Polyclonal
Product Videos
Custom Field
Size 100 µl
Target PDK1
Conjugate HRP
Research Area Cell Signaling | Cytoskeleton | Microfilaments | Actin | Cell Signaling | Protein Phosphorylation | Serine / Threonine Kinases
Alternative Name PDK1 Antibody, PDPK1 Antibody, PDPK1_HUMAN Antibody, PDPK2 Antibody, PkB kinase Antibody, PkB kinase like gene 1 Antibody, PkB like 1 Antibody, PRO0461 Antibody, Protein kinase Antibody3 phosphoinositide dependent protein kinase 1 Antibody, 3-phospho
Category Antibodies
Product Type Polyclonal Antibody
Immunogen Synthetic peptide of human PDK1 (AA223-238)
Immunogen Species Human
Applications WB, AM
Host Rabbit
Isotype N/A
Species Reactivity Human, Mouse
Antibody Dilution WB (1:250); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Concentration N/A
Specificity Detects ~63kDa.
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis A 1:250 dilution of SPC-1186 was sufficient for detection of PDK1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm, Nucleus, Cell Membrane, Cell Junction, Focal Adhesion
Tissue Specificity Expressed ubiquitously with highest expression in blood vessels and the brain. The Tyr-9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast.
Accession Number NP_002601
Gene ID 5170
Swiss-Prot O15530
Field of Use For in vitro research use only.
