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Product Description
Mouse Anti- Phosphotyrosine Monoclonal IgG1
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Size 100 µg
Other Name Phosphotyrosine Antibody: Biotin
Target Phosphotyrosine
Conjugate Biotin
Research Area Cell Signaling, Post-translational Modifications, Phosphorylation
Alternative Name(s) PhosphoTyrosine (pY) Antibody, PhosphoTyrosine (pY) Antibody
Product Type Monoclonal Antibody
Clone Number G104
Immunogen Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3'-dimentrylaminopropyl) carbodiimide
Immunogen Species N/A
Applications WB, IHC, ICC/IF, IP
Host Mouse
Isotype IgG1
Species Reactivity All
Antibody Dilution WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Specificity Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphothreonine.
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-174 was sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Cellular Localization N/A
Accession Number N/A
Gene ID N/A
Swiss-Prot N/A
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
