Product Description
This gene encodes a 190 kDa nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The BRCA1 gene contains 22 exons spanning about 110 kb of DNA. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants have been described. A related pseudogene, which is also located on chromosome 17, has been identified.
Biovision | A2157 | Anti-BRCA1 Antibody DataSheet
Antibody Target: BRCA1
Target Alternative Name: IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
Tag Line: Affinity purified rabbit polyclonal antibody to detect the expression of BRCA1 in human, mouse and rat samples
Category: Primary antibody
Host: Rabbit
Isotype: IgG
Species Reactivities: Rat,Mouse,Human
Immunogen Sequence: KLH-conjugated synthetic peptide targeting a sequence within the C-term region of human BRCA1
Accession #: P38398
Gene ID: 672
Appearance: Colorless liquid
Form: Liquid
Concentration:
Formulation: In 0.42% Potassium phosphate; 0.87% NaCl; pH 7.3; 30% glycerol; and 0.01% sodium azide
Purification: Affinity purified
Application: Immunohistochemistry (IHC),Western Blotting
Positive Control:
Application And Usages: WB 1:500 - 1:1000, IHC 1:100 - 1:200
Country of Animal Origin: USA
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: TRUE
Immunocytochemistry Verified: FALSE
Immunofluorescence Verified: FALSE
Immunoprecipitation Verified: FALSE
FACS Verified: FALSE
ELISA Verified: FALSE
ChIP Verified: FALSE
Dot Blot Verified: FALSE
Flow Cytometry Verified: FALSE