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Product Description
Programmed death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PD-1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-?. PD-L2 expression is more restricted and is expressed mainly by dendritic cells. Elevated levels of PD-1 inhibit the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-? by suppressing the activation and transduction of PI3K/AKT pathway. In addition, co-ligation of PD-1 inhibits BCR-mediating signal by de-phosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1 ligand results in reduced T cell proliferation and IFN-? secretion.
Biovision | A1996 | Anti-PD-1 Biotinylated Antibody DataSheet
Antibody Target: Biotinylated PD-1
Target Alternative Name: CD279, PDCD1, PD1, hPD-1, hPD-l, SLEB2
Tag Line: Affinity purified rabbit polyclonal antibody to detect biotinylated PD-1 expression in human, mouse and rat samples
Category: Immune Checkpoint
Host: Rabbit
Isotype: IgG
Species Reactivities: Rat, Mouse, Human
Immunogen Sequence: Human PD-1 recombinant protein
Accession #: Q15116
Gene ID: 5133
Appearance: Colorless liquid
Form: Liquid
Concentration:
Formulation: In PBS with 30% glycerol, 0.5% BSA, 0.5mM EDTA, 0.03% Proclin, pH 7.2
Purification: Affinity purified
Application: Western Blotting, ELISA
Positive Control:
Application And Usages: WB 1:50-200, ELISA 1:500
Country of Animal Origin: USA
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: True
Immunocytochemistry Verified: False
Immunofluorescence Verified: False
Immunoprecipitation Verified: False
FACS Verified: False
ELISA Verified: True
ChIP Verified: False
Dot Blot Verified: False
Flow Cytometry Verified: False
