Cell Signaling | Protein Phosphorylation | Serine / Threonine Kinases | PKC | Cardiovascular System | Heart | Hypertrophy
Alternative Name:
cAMP dependent protein kinase catalytic subunit alpha isoform 1 Antibody, cAMP-dependent protein kinase catalytic subunit alpha Antibody, KAPCA_HUMAN Antibody, PKA C alpha Antibody, PKA C-alpha Antibody, PKACA Antibody, PRKACA Antibody, Protein kinas
Category:
Antibodies
Product Type:
Polyclonal Antibody
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Thr196 and Thr198of human PRKACA (AA193-201)
Immunogen Species:
Human
Applications:
WB, AM
Host:
Rabbit
Isotype:
N/A
Species Reactivity:
Human
Antibody Dilution:
WB (1:250); optimal dilutions for assays should be determined by the user.
Purification:
Peptide Affinity Purified
Storage Buffer:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Concentration:
N/A
Specificity:
Detects 40.59 kDa.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:250 dilution of SPC-1066 was sufficient for detection of PRKACA (pThr196 + pThr198) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Certificate of AnalysisA 1:250 dilution of SPC-1066 was sufficient for detection of PRKACA (pThr196 + pThr198) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Tissue SpecificityIsoform 1 is ubiquitous. Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids.