A phospho-specific peptide corresponding to residues surrounding Tyr220 and Ser221 of human RSK1 (AA217-224)
Immunogen Species:
Human
Applications:
WB, AM
Host:
Rabbit
Isotype:
N/A
Species Reactivity:
Human
Antibody Dilution:
WB (1:250); optimal dilutions for assays should be determined by the user.
Purification:
Peptide Affinity Purified
Storage Buffer:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Concentration:
N/A
Specificity:
Detects 82.723 kDa.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:250 dilution of SPC-1079 was sufficient for detection of RSK1 (pTyr220+pSer221) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization:
Nucleus, Cytoplasm
Tissue Specificity:
Widely expressed with higher expression in lympocytes, granulocytes, leukocytes, macrophages, blood, and cerebrospinal fluid cells.
Certificate of AnalysisA 1:250 dilution of SPC-1079 was sufficient for detection of RSK1 (pTyr220+pSer221) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular LocalizationNucleus, Cytoplasm
Tissue SpecificityWidely expressed with higher expression in lympocytes, granulocytes, leukocytes, macrophages, blood, and cerebrospinal fluid cells.