Product Description
Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-? and form a heterodimer which is part of the ISGF3 transcription factor complex. Altho?gh early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3? appears to be activated by both while Stat3? is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
Biovision | 3470R | Stat3 Antibody DataSheet
Antibody Target: STAT3
Target Alternative Name: Signal transducer and activator of transcription 3, stat -3, STAT3, STAT -3, STAT 3, APRF , FLJ20882 , mgC16063
Tag Line:
Category: Stat
Host: Rabbit
Isotype: Rabbit IgG
Species Reactivities: Human, mouse, rat
Immunogen Sequence: Synthetic peptide surrounding amino acid 73 of rat Stat3
Accession #: AAA19452.1
Gene ID: 20848
Appearance: Colorless liquid
Form: Liquid
Concentration: 0.5 mg/ml
Formulation: 30 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Purification: Affinity purified
Application: Western blot
Positive Control:
Application And Usages: Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~97 kDa Stat3 in samples from human, mouse, and rat origins. Reactivity to other species has not been tested.
Country of Animal Origin: China
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: Verified
Immunocytochemistry Verified: Not Verified
Immunofluorescence Verified: Not Verified
Immunoprecipitation Verified: Not Verified
FACS Verified:
ELISA Verified: Not Verified
ChIP Verified: Not Verified
Dot Blot Verified:
Flow Cytometry Verified: Not Verified