Product Description
Rabbit Anti-Human Vimentin Polyclonal
Custom Field
Size 100 µl
Target Vimentin
Conjugate APC/Cy7
Research Area Neuroscience | Neurogenesis | Cell Signaling | Cytoskeleton | Intermediate Filaments | Vimentin | Neuroscience | Cell Markers | Neuron Markers | Neural Stem Cell Markers | Stem Cells | Lineage Markers | Endoderm | Cancer | Tumor Biomarkers | Neuroreg
Alternative Name CTRCT30 Antibody, Epididymis luminal protein 113 Antibody, HEL113 Antibody, VIM Antibody, VIME_HUMAN Antibody, Vimentin Antibody
Category Antibodies
Product Type Polyclonal Antibody
Immunogen Synthesized peptide derived from Internal of human vimentin.
Immunogen Species Human
Applications WB, IHC, ELISA
Host Rabbit
Isotype IgG
Species Reactivity Human, Mouse, Rat
Antibody Dilution WB (1:1000), IHC (1:100), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Concentration N/A
Specificity Detects ~53kDa, endogenous levels of total vimentin protein.
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-1338 was sufficient for detection of Vimentin in 10 µg of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm
Tissue Specificity Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Accession Number NP_003371.2
Gene ID 7431
Swiss-Prot P08670
Field of Use For in vitro research use only.