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Product Description
EnzyChrom™ Catalase Assay Kit
Application: For quantitative determination of catalase activity and evaluation of drug effects on catalase activity.
Key Features:
- Sensitive and accurate. Use 10 μL sample. Linear detection range 0.2 to 5 U/L catalase activity.
- Simple and Convenient. The procedure involves adding a Substrate to sample, incubation for 30 min, followed by a Detection Reagent and reading the optical density or fluorescence intensity.
Method: OD570nm, or FL530/585nm
Samples: Serum, plasma, urine, saliva, cell culture etc
Species: All
Size: 100 tests
Detection Limit: 0.2 U/L
Shelf Life: 6 months
More Details:
CATALASE (EC 1.11.1.6), is an ubiquitous antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (H2O2) to water and oxygen. By preventing excessive H2O2 build up, catalase allows important cellular processes which produce H2O2 as a byproduct to occur safely. Deficiency in catalase activity has been associated with grey hair and peroxisomal disorder acatalasia.
Simple, direct and high-throughput assays for catalase activity find wide applications. BioAssay Systems improved assay directly measures catalase degradation of H2O2 using a redox dye. The change in color intensity at 570nm or fluorescence intensity (λex/em = 530/585nm) is directly proportional to the catalase activity in the sample.
