Product Description
EnzyChrom™ Glutathione Reductase Kit
Application: For quantitative determination of glutathione reductase activity.
Key Features:
- Fast and sensitive. Linear detection range (20 μL sample): 0.4 to 50 U/L for 20 min reaction. Detection Limit of 0.4 U/L.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method: OD412nm
Samples: Plasma, serum, tissue and culture media etc
Species: All
Size: 100 tests
Detection Limit: 0.4 U/L
Shelf Life: 6 months
More Details:
GLUTATHIONE REDUCTASE (GR) reduces oxidized glutathione (GSSG) to the reduced sulfhydryl form GSH which is an important cellular antioxidant. A high GSH/GSSG ratio is important for protection against oxidative stress. Thus, measurement of GR activity is used as indicator for oxidative stress.
BioAssay Systems' non-radioactive, colorimetric GR assay is designed to accurately measure GR activity in biological samples with a method that utilizes Ellman?s method in which DTNB reacts with the GSH generated from the reduction of GSSG by the GR in a sample to form a yellow product (TNB2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to GR activity in the sample.
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