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Product Description
EnzyChrom™ Lactose Assay Kit
Application: For quantitative determination of lactose and evaluation of drug effects on lactose metabolism.
Key Features: Use as little as 20 μL samples. Linear detection range in 96-well plate: 17 to 2000 μM lactose for colorimetric assays and 6 to 100 μM for fluorimetric assays.
Method: OD570nm, or FL530/585nm
Samples: Biological (milk), food and agriculture etc
Species: All
Size: 100 tests
Detection Limit: 6 μM
Shelf Life: 6 months
More Details:
Lactose (C12H22O11), also called milk sugar, is a disaccharide that consists of β-D-galactose and α/β-D-glucose through a β1-4 glycosidic linkage. Lactose is the major sugar and makes up 2–8% of milk. Simple, direct and high-throughput assays for lactose determination find wide applications.
BioAssay Systems assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample.
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