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Product Description
EnzyFluo™ NAD/NADH Assay Kit
Application: For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. Direct NAD/NADH Assays in 96-Well PlateNEW!!!
Key Features:
- Sensitive and accurate. Detection limit of 0.02 μM and linearity up to 1 μM NAD+/NADH in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Method: F530/585nm
Samples: Cell, tissue extracts etc Direct NAD/NADH Assays in 96-Well PlateNEW!!!
Species: All
Size: 100 tests
Detection Limit: 0.02 μM
Shelf Life: 6 months
More Details:
Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue.
BioAssay Systems EnzyFluo™ NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the NAD+/NADH concentration in the sample.
This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient method to measure NAD, NADH and their ratio.
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