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Product Description
pNPP Phosphatase Assay Kit
Application: For quantitative determination of phosphatase activity.
Key Features:
- High sensitivity and wide linear range. The detection limit is generally 3 ng phosphatase or below.
- Homogeneous and simple procedure. No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes.
- Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
Method: OD405nm
Samples: Protein phosphatases
Species: All
Size: 500 tests
Detection Limit: 3 ng
Shelf Life: 12 months
More Details:
p-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at 405 nm on a spectrophotometer.
This homogeneous "mix-and-measure" assay involves simply adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37°C.
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