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Application: For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism.
Key Features:
Method: OD565nm
Samples: Biological samples (e.g. plasma, serum, tissue and culture media).
Species: All
Size: 100 tests
Detection Limit: 0.5 U/L
Shelf Life: 6 months
More Details:
GLUCOSE DEHYDROGENASE (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway. BioAssay Systems' non-radioactive, colorimetric GDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.