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  • QuantiChrom Isocitrate Dehydrogenase Assay Kit

    • QuantiChrom™ Isocitrate Dehydrogenase Assay Kit

    NULL587.00

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    SKU:
    65-DIDH-100
    Availability:
    Usually ships in 5 working days
    Application:
    Quantitative colorimetric kinetic determination of isocitrate dehydrogenase activity and evaluation of drug effects on its metabolism
    Method:
    OD565nm
    Samples:
    plasma, serum,tissue and culture media etc
    Species:
    all
    Procedure:
    30 min.Size= 100 tests
    Storage:
    -20°C
    Shipping:
    Room Temperature
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    Product Description

    QuantiChrom™ Isocitrate Dehydrogenase Assay Kit

     

    Application: For quantitative colorimetric kinetic determination of isocitrate dehydrogenase activity and evaluation of drug effects on its metabolism.

    Key Features:

    • Fast and sensitive. Linear detection range (20 μL sample): 0.1 to 100 U/L for 30 min reaction.
    • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

    Method: OD565nm

    Samples: Plasma, serum,tissue and culture media etc

    Species: All

    Size: 100 tests

    Detection Limit: 0.1 U/L

    Shelf Life: 6 months

    More Details:

    ISOCITRATE DEHYDROGENASE (IDH) is an enzyme which catalyzes the interconversion of isocitrate and a-ketoglutarate. There are three IDH isoforms: IDH3 uses the cofactor NAD+ and catalyzes the third step in the citric acid cycle, while IDH1 and IDH2 use the cofactor NADP+ and catalyze the same reaction outside the citric acid cycle. This kit measures the activity of the NADP+ isoforms.

    Mutations in IDH1 and IDH2 have been linked with various brain tumors and acute myeloid leukemia. BioAssay Systems' non-radioactive, colorimetric IDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

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    Application Quantitative colorimetric kinetic determination of isocitrate dehydrogenase activity and evaluation of drug effects on its metabolism
    Method OD565nm
    Samples plasma, serum,tissue and culture media etc
    Species all
    Procedure 30 min.Size= 100 tests
    Storage -20°C
    Shipping Room Temperature

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