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Product Description
Biovision | K2023 | Trypsin Activity Assay Kit (Fluorometric) DataSheet
Trypsin (EC 3.4.21.4) is a serine protease that is expressed in bacteria, fungi and mammals. Trypsin cleaves peptide chains at the carboxyl side of the amino acids namely arginine and lysine. In mammals, trypsin is secreted from the pancreas as an inactive precursor (trypsinogen) and is processed to its active form in the small intestine, where it plays an essential role in protein hydrolysis and absorption. Activation of trypsin in pancreas can lead to a series of events thereby resulting in pancreatitis. Trypsin is therefore a useful biomarker for pancreatitis. Additionally, monitoring trypsin activity plays a central role in biochemical, pharmaceutical and has clinical applications. BioVision’s Trypsin Activity Assay Kit provides a simple, rapid way to detect trypsin activity in a wide variety of biological samples. In this kit, trypsin cleaves a synthetic substrate thereby releasing a fluorophore (AMC), which can be easily quantified at Ex/Em = 380/460 nm. By pretreating samples at 60ºC for 20 min, the kit can differentiate the trypsin activity from other structurally similar enzymes such as chymotrypsin and tryptase. The assay is specific, sensitive and can detect as low as 2 µU of trypsin activity in samples.
Alternate Name : Trypsin Activity Kit, Trypsin Activity Kit (Fluorometric), Trypsin Activity Assay Kit, Trypsin Activity Kit (Fluorometric), Trypsin Activity Assay Kit (Fluorometric), Trypsin Assay Kit (Fluorometric), Trypsin Fluorometric ActivityKit, Trypsin Activity, Trypsin Fluorometric Kit, Trypsin Fluorometric Assay Kit, Trypsin Activity Assay, Trypsin Activity Assay, Trypsin Fluorometric Activity Assay, Trypsin Fluorometric Assay, Trypsin Fluorometric Activity Assay Kit
Tag Line : to detect trypsin activity
Summary : BioVision’s Trypsin Activity Assay Kit provides a simple, rapid way to detect trypsin activity in a wide variety of biological samples. In this kit, trypsin cleaves a synthetic substrate thereby releasing a fluorophore (AMC), which can be easily quantified at Ex/Em = 380/460 nm. By pretreating samples at 60ºC for 20 min, the kit can differentiate the trypsin activity from other structurally similar enzymes such as chymotrypsin and tryptase. The assay is specific, sensitive and can detect as low as 2 µU of trypsin activity in samples.
Detection Method : Fluorescence (Ex/Em = 380/460 nm)
Sample Type : Tissue Homogenates: Intestine, Biological Fluids: Serum
Species Reactivity : All
Applications : Measurement of trypsin activity in Biological Fluids, Tissues
Features and Benefits : Can detect as low as 2 µU of trypsin activity in samples, Specific and sensitive, Rapid way to detect trypsin activity in a wide variety of biological samples, Simple
