Product Description
Biovision | K175 | 3 to 5 Exonuclease Activity Assay (Fluorometric) DataSheet
Exonucleases remove a single nucleotide or an oligonucleotide at the 5 or 3 end. Exonuclease, such as Three Prime Repair Exonuclease 1 (TREX1), plays an important role in proofreading of DNA amplification and removal of damaged DNA. Deficiency of TREX1 enzyme has been shown to trigger autoimmune disease, Aicardia-Goutieres syndrome. TREX1 expression has been reported to correlate with cervical cancer cell growth and disease progression. In addition, 3 exonucleases are often used as tools in molecular biology such as site-directed mutagenesis and the generation of single stranded DNA probe. BioVisions 3 to 5 Exonuclease Activity Assay Kit provides a quick and easy method for monitoring 3 exonuclease activities in a wide variety of samples. In this assay, exonuclease digests the provided DNA probe, producing a strong fluorescent signal (Ex/Em= 304/369nm). The kit is simple, sensitive, high-throughput adaptable and can detect as low as 0.2 µU of exonuclease activity.
Alternate Name : 3 to 5 Exonuclease Assay (Fluorometric), 3 to 5 Exonuclease Activity Assay, 3 to 5 Exonuclease Activity Assay, 3 to 5 Exonuclease Activity Assay (Fluorometric), 3 to 5 Exonuclease Activity (Fluorometric), 3 to 5 Exonuclease Fluorometric Activity Assay, 3 to 5 Exonuclease Fluorometric Assay, 3 to 5 Exonuclease Assay, Exonuclease Activity Assay (Fluorometric)
Tag Line : For monitoring 3 exonuclease activity in a wide variety of samples
Summary : BioVisions 3 to 5 Exonuclease Activity Assay Kit provides a quick and easy method for monitoring 3 exonuclease activities in a wide variety of samples.
Detection Method : Fluorescence (Ex/Em = 304/369 nm)
Sample Type : Animal tissue, Mammalian and bacterial cell culture
Species Reactivity : All
Applications :
Features and Benefits : Sensitive, High-throughput adaptable, Detection limit as low as 0.2 µU of exonuclease activity, Simple