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Product Description
Biovision | E4853 | PGFM ELISA Kit DataSheet
In many species, uterine and placental Prostaglandin F2alpha (PGF2a) is involved in the regulation of reproductive and pregnancy-related processes such as embryonic development, initiation of parturition, and resumption of ovarian activity. PGFM has been suggested as a useful non-invasive marker of pregnancy when measured in both urine and fecal samples. BioVision’s PGFM ELISA kit is a competitive ELISA assay for the quantitative measurement of PGFM in fecal extracts, urine, serum and plasma samples. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A PGFM-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to PGFM to each well. After incubation the plate is washed and substrate is added. The substrate reacts with the bound PGFM -peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength.
Alternate Name :
Tag Line : A competitive ELISA kit for the quantitative measurement of PGFM in fecal, urine, serum, plasma and tissue culture samples.
Summary :
Detection Method : Absorbance (450 nm)
Sample Type : Fecal Extracts,Plasma,Cell and tissue culture supernatants,Serum,Urine
Species Reactivity : All
Applications : This ELISA kit is used for in vitro quantitative determination of PGFM
Features and Benefits : Detection Limit: 46.2 pg/mL,Sensitivity:20.8 pg/mL,Detection Range: 50- 3200 pg/mL,This ELISA kit is used for in vitro quantitative determination of PGFM
