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Product Description
Biovision | K2094 | Nitric Oxide Synthase (NOS) Activity Assay Kit II (Colorimetric) DataSheet
Nitric oxide synthases (EC 1.14.13.39, NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. NO plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes the five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS including endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in the central nervous system and participates in cell communication and information storage. eNOS produces NO in the blood vessels and is involved in the regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo during oxidative stress and large amounts of NO are produced as part of body’s defense mechanism. BioVision’s Nitric Oxide Synthase Activity Assay Kit II provides an accurate and convenient method to assay NOS activity in a variety of sample types. In this assay, NO generated by NOS undergoes a series of reactions and then reacts with Griess Reagent 1 and 2 to generate a colored product with a strong absorbance at 540 nm. The assay is simple, fast, sensitive and is high-throughput adaptable.
Alternate Name : NOS Activity Assay Kit II NOS Activity Assay Kit II (Colorimetric) Nitric Oxide Synthase Activity Assay Kit II Nitric Oxide Synthase (NOS) Activity Assay Kit II
Tag Line : An accurate and convenient method to assay NOS activity in a variety of sample types.
Summary : BioVision’s Nitric Oxide Synthase Activity Assay Kit II provides an accurate and convenient method to assay NOS activity in a variety of sample types.
Detection Method : Absorbance at 540 nm
Sample Type : Tissue or cell extracts Purified recombinant protein
Species Reactivity :
Applications : Detection of NOS activity in various sample types
Features and Benefits : The assay is simple, fast, sensitive and is high-throughput adaptable. An accurate and convenient method to assay NOS activity in a variety of sample types.
